Truncated equine LH beta and asparagine(56)-deglycosylated equine LH alpha combine to produce a potent FSH antagonist

SOAR Repository

Show simple item record

dc.contributor.author Butnev, Vladimir Y. en_US
dc.contributor.author Singh, V. en_US
dc.contributor.author Nguyen, Van T. en_US
dc.contributor.author Bousfield, George R. en_US
dc.date.accessioned 2012-01-24T17:48:25Z
dc.date.available 2012-01-24T17:48:25Z
dc.date.issued 2002-03 en_US
dc.identifier 11874703 en_US
dc.identifier AG15428/ DK52383 en_US
dc.identifier 0375363 en_US
dc.identifier JOE04323 en_US
dc.identifier.citation The Journal of endocrinology. 2002 Mar; 172(3): 545-55. en_US
dc.identifier.issn 0022-0795 en_US
dc.identifier.uri http://dx.doi.org/10.1677/joe.0.1720545
dc.identifier.uri http://hdl.handle.net/10057/4123
dc.description Click on the DOI link below to access the article (may not be free). en_US
dc.description.abstract Hybrid hormone preparations were prepared by combining intact and Asn(56)-deglycosylated (N(56)dg) equine (e) LH or FSH alpha subunit preparations with truncated, des(121-149)eLH beta (eLH beta t), immunopurified, intact eLH beta or equine chorionic gonadotropin beta (eCG beta) preparations, and eFSH beta. The LH receptor-binding potencies of N(56)dg-eLH alpha:eLH beta t and N(56)dg-eFSH alpha:eLH beta t hybrids were equivalent to that of eLH; however, both N(56)dg-alpha preparations were only 3-4% as active as eLH in the rat testis Leydig cell bioassay. In the granulosa cell FSH bioassay, eLH alpha:eLH beta t stimulated progesterone synthesis and induced aromatase activity, while N(56)dg-eLH alpha:eLH beta t was completely inactive at doses up to 5 microg. N(56)dg-eLH alpha:eLH beta t inhibited progesterone production and aromatase induction elicited by 0.3 ng eFSH or 2 ng human (h) FSH. The inhibitory activities of N(56)dg-eLH alpha:eLH beta and N(56)dg-eCG alpha:eLH beta t were only 10% that of N(56)dg-eLH alpha:eLH beta t. N(56)dg-eLH alpha:eCG beta did not inhibit progesterone synthesis stimulated by eFSH at all and appeared to further stimulate aromatase induction at the highest dose tested. Preincubation of N(56)dg-eLH alpha:eLH beta and N(56)dg-eLH alpha:eLH beta t for 72 h at 37 C resulted in no loss of FSH receptor-binding activity. Preincubation resulted in 50% loss of receptor-binding activity by the eFSH preparation due to subunit dissociation, while 88% of N(56)dg-eLH alpha:eFSH beta activity was lost following 72 h, 37 C preincubation. While alpha Asn(56) oligosaccharide had no effect on eLH beta hybrid stability, it did contribute to the stability of the eFSH heterodimer. en_US
dc.description.sponsorship NIA NIH HHS/ NIDDK NIH HHS en_US
dc.language.iso eng en_US
dc.publisher BioScientifica Ltd. en_US
dc.relation.ispartofseries The Journal of endocrinology en_US
dc.source NLM en_US
dc.subject Research Support, U.S. Gov't, Non-P.H.S. en_US
dc.subject Research Support, U.S. Gov't, P.H.S. en_US
dc.subject.mesh Animals en_US
dc.subject.mesh Dimerization en_US
dc.subject.mesh Dose-Response Relationship, Drug en_US
dc.subject.mesh Female en_US
dc.subject.mesh Follicle Stimulating Hormone/antagonists & inhibitors en_US
dc.subject.mesh Glycoprotein Hormones, alpha Subunit/metabolism en_US
dc.subject.mesh Granulosa Cells/drug effects en_US
dc.subject.mesh Horses en_US
dc.subject.mesh Luteinizing Hormone/chemistry en_US
dc.subject.mesh Radioligand Assay/methods en_US
dc.subject.mesh Rats en_US
dc.subject.mesh Recombinant Proteins/metabolism en_US
dc.subject.mesh Glycoprotein Hormones, alpha Subunit/metabolism en_US
dc.subject.mesh Granulosa Cells/metabolism en_US
dc.subject.mesh Luteinizing Hormone/metabolism* en_US
dc.title Truncated equine LH beta and asparagine(56)-deglycosylated equine LH alpha combine to produce a potent FSH antagonist en_US
dc.type Article en_US
dc.description.version peer reviewed en_US
dc.rights.holder Copyright @ 2002 Society for Endocrinology en_US

Files in this item

Files Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record

Search SOAR

Advanced Search


My Account