Active targeted drug delivery occurs when a drug is associated with a biodegradable polymer and a bio-targeting compound and administered to the site of interest. In this study, these nanocomposite spheres are characterized using various techniques. In-vitro characterization of the nano-spheres containing therapeutic agent and fluorescence molecules are accomplished by culturing live cells in microscopic cells, and introducing the nano-spheres. In-vivo studies are conducted using immune-deficient mice. Other characteristics such as release rates, percent entrapment efficiency and size are studied with UV-Vis spectroscopy, TGA, and TEM.