The research presented here details the development of two separate methods for ESI-MS and LC. The first chapter gives an introduction to the first of the methods presented here, the detection of ketamine in alcoholic drinks. The second chapter gives a brief introduction into the relevance of developing a method for investigating clomiphene in biological samples. The third and fourth chapters outline the experimental parameters for the ketamine and clomiphene detection methods, respectively. Included are the parameters used for the mass spectrometer, liquid chromatography, and UV/Visible absorbance spectroscopy as well as the methods for extraction and sample work up. Chapter five outlines the development of a method for detecting and quantifying the date rape drug Ketamine HCl in alcoholic drink matrices. The sixth chapter outlines the development of LC and MS methods for detecting and quantifying clomiphene citrate in fetal calf serum and the initial results for the extraction and detection of clomiphene citrate in meconium. Chapters seven and eight present the conclusions for the ketamine and clomiphene studies, respectively, with directions for future studies.

Thesis (M.S.)--Wichita State University, College of Liberal Arts and Sciences, Dept. of Chemistry