Transport of tryptophan into SH-SY5Y neuronal cells

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dc.contributor.author	Kollalpitiya, Konara Yamuna
dc.contributor.author	Wimalasena, Kandatege
dc.date.accessioned	2008-06-05T18:57:11Z
dc.date.available	2008-06-05T18:57:11Z
dc.date.issued	2008-04-25
dc.identifier.citation	Kollalpitiya,Yamuna, Wimalasena, Kandatege(2008). Transport of tryptophan into SH-SY5Y neuronal cells. In Proceedings: 4th Annual Symposium: Graduate Research and Scholarly Projects. Wichita, KS: Wichita State University, p.149-150	en
dc.identifier.uri	http://hdl.handle.net/10057/1403
dc.description	Paper presented to the 4th Annual Symposium on Graduate Research and Scholarly Projects (GRASP) held at the Hughes Metropolitan Complex, Wichita State University, April 25, 2008.	en
dc.description	Research completed at the Department of Chemistry, Fairmount College of Liberal Arts and Sciences	en
dc.description.abstract	The human neuroblastoma cell line SH-SY5Y is a commonly used catecholaminergic model in studies related to neurotoxicity of various substances, oxidative stress, and neurodegenerative diseases. Specific transport of monoamine neurotransmitter precursors i.e. corresponding aromatic amino acids, into these cells is essential for cellular metabolism. Serotonin (5-hydroxytryptamine) is a neurotransmitter synthesized in the brain that is implicated in many brain disorders including Schizophrenia and manic depression. Serotonin is synthesized from tryptophan in serotonergic neurons. In the present study we have examined the uptake of L-tryptophan into SH-SY5Y cells by reverse phase high performance liquid chromatography coupled with UV detection. This study shows that SH-SY5Y cells possess significant capacity to take up extracellular tryptophan. The tryptophan uptake was inhibited by a specific inhibitor of system L, a transport system present in mammalian cells serves for neutral amino acids, providing evidence that tryptophan enters the cells via transport system L. Both undifferentiated and 12-O-tetradecanoly-phorbol-12-acetate (TPA) differentiated SH-SY5Y cells show similar tryptophan uptake efficiencies.	en
dc.format.extent	178884 bytes
dc.format.mimetype	application/pdf
dc.language.iso	en_US	en
dc.publisher	Wichita State University. Graduate School.	en
dc.relation.ispartofseries	GRASP	en
dc.relation.ispartofseries	v.4	en
dc.title	Transport of tryptophan into SH-SY5Y neuronal cells	en
dc.type	Conference paper	en